Intracytoplasmic Morphologically Sperm Injection (IMSI)

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Intra-cytoplasmic Morphologically Selected Sperm Injection (IMSI)


IMSI is one of the techniques that increase the success rate of IVF. 
The sperm is selected under a high power light microscope (enhanced by digital imaging) to magnify the sperm sample over 6000 times. With this microscope we can see the internal morphology of the sperm and discard those with abnormalities. According to the WHO a mature sperm should ideally have an oval head with a length of between 4 and 5 microns and a width between 2.5 and 3.5 microns. One healthy sperm is injected into each egg.


The embryologist selects the best looking sperm using a high-power and accurate optical microscope (equipped with digital imaging) to enlarge the sperm sample more than 6000 times, which allows the detection of abnormal sperm. This technique is used when the number of sperm abnormalities is large, so one normal sperm is injected into each egg.


IMSI is a method to increase the success rate of the IVF procedure, by selecting the sperm without morphological abnormalities using a high-definition microscope that allows a magnification of the sperm up to 6000 times and this exceeds the magnification capacity usually used in laboratories of reproduction whose magnification strength reaches only 400 times. Thus, detailed head imaging allows us to assess the gaps that are likely to exist. In this way pre-selection of sperm before fertilization is done using microscopic injection and only the best sperm is used. Using this microscope, allows us to view and examine the internal morphology of sperms (including the nucleus) and ignore those with abnormalities. This permits us to choose sperms without morphological problems.


The selected sperm is injected into the egg's cytoplasmic membrane. One sperm is inserted into each egg.
The use of this technique improves the chances of success and the opportunities for fertilizing eggs in the laboratory and can be used in cases of repeated failures, i.e. after several failed attempts of IVF or in cases where the sperm has a very high number of abnormalities.
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